Assessment of Oxidative Stress in Early and Late Onset Pre-Eclampsia among Ghanaian Women

Abstract

Backgound: Pre—eclampsia is a multi;system pregnancy-related disorder with multiple theories regarding its aetiology resulting in lack of reliable screening tests and well-established measures for primary prevention. However, oxidative stress is increasingly being implicated in the pathogenesi of pre-eclampsia although conflicting findings have beei reported. Aim: To determine and compare the levels of oxidative stress in early and late onset pre-eclampsia by measuring urinary excretion of isoprostane and total antioxidant power (TAP) in a cohort of pre-eclamptic women at Korle Bu Teaching Hospital. Methodology: This was a cross-sectional study conducted at Korle-Bu Teaching Hospital, Accra, Ghana involving pre eclamptic women between the ages 18 and 45 years who gave written informed consent. Urinary isoprostane levels were determined using an enzyme-linked immunosorbent assay (ELISA) kit whereas the Total Anti-oxidant Power in Urine samples was determined using Total Antioxidant Power Colorimetric Microplate Assay kit. The data obtained were analyzed using MEGASTAT statistical software package. Results: We included 102 pre-eclamptic women comprising 68 (66.7%) and 34 (33.3%) with early-onset and late-onset pre-eclampsia respectively. There were no statistically significant differences between the mean maternal age haematological indices, serum ALT, AST, ALT, albumin, urea, creatinine uric acid and total protein at the time of diagnosis The mean gestational age at diagnosis of early and late onset pre-eclampsia were 31.65 ± 0.41 and 38.03 ± 0.21 respectively (p < 0.001). Also, there were statistically significant differences between the diastolic blood pressure (BP) systolic BP and mean arterial pressure (MAP) at diagnosis of pre-eclampsia in the two categories. The mean urinari isoprostane excretion was significantly higher in the early onset pre-eclamptic group (3.04 ± 0.34 ng/mg Cr) compared ti that of the late onset pre-eclamptic group (2.36 ± 0.45 ng/mg Cr), (p=0.019). Urinarytotal antioxidant power (TAP) in earli onset PE (1.6410.06) was lower but not significantlydifferent from that of late onset PE (1.74 ± 0.09) with p = 0.369. Conclusion: Significantly increased urinary isoprostane excretion was detected in early onset pre-eclampsia compared ti late onset pre-eclampsia, suggestive of increased oxidative stress in the former. However, there was no significan difference in total anti-oxidant power between the two categories of pre~ec|ampsia women although there was 1 tendency of reduced total antioxidant power in the women with early onset pre-ecalmpsia.