University of Jos Institutional Repository >
Medical Sciences >
Medical Laboratory Sciences >

Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/1684

Title: Comparison of Enzyme-Linked Immunospot Assay and Tuberculin Skin Test in Healthy Children Exposed to Mycobacterium Tuberculosis
Authors: Hill, Philip C.
Brookes, Roger H.
Adetifa, Ifedayo M.O.
Fox, Annette
Jackson-Sillah, Dolly
Lugos, Moses D.
Donkor, Simon A.
Marshall, Roger J.
Howie, Stephen R.C.
Corrah, Tumani
Jeffries, David J.
Adegbola, Richard A.
McAdam, Keith P.W.J.
Keywords: ELISPOT
Issue Date: May-2006
Publisher: Pediatrics
Series/Report no.: Vol. 117;No. 5; Pp 1542 - 1548
Abstract: OBJECTIVE. To compare the enzyme-linked immunospot (ELISPOT) assay with the tuberculin skin test (TST) in children for the diagnosis of Mycobacterium tuberculosis infection in the Gambia. METHODS.We divided child contacts of sputum smear-positive tuberculosis cases into 3 age categories ( 5, 5–9, and 10–14 years) and assessed agreement between the 2 tests plus their relationship to prior Bacille Calmette-Guerin (BCG) vaccination. We categorized a child’s level of M tuberculosis exposure according to where he/she slept relative to a case: the same room, same house, or a different house. The relationship between exposure and test result was assessed by multiple logistic regression. RESULTS. In child contacts of 287 cases, 225 (32.5%) of 693 were positive by TST and 232 (32.3%) of 718 by ELISPOT. The overall agreement between tests was 83% and the discordance was not significant. Both tests responded to the M tuberculosis exposure gradient in each age category. The percentage of those who were TST positive/ELISPOT negative increased with increasing exposure. At the lowest exposure level, the percentage of ELISPOT-positive children who were TST negative was increased compared with the highest exposure level. Neither test had evidence of false positive results because of BCG. CONCLUSIONS. In Gambian children, the ELISPOT is slightly less sensitive than the TST in the diagnosis of M tuberculosis infection from recent exposure, and neither test is confounded by prior BCG vaccination. Evidence of reduced TST sensitivity in subjects with the lowest known recent M tuberculosis exposure suggests that, when maximal sensitivity is important, the 2 tests may be best used together.
URI: http://hdl.handle.net/123456789/1684
Appears in Collections:Medical Laboratory Sciences

Files in This Item:

File Description SizeFormat
1542.full.pdf362.1 kBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback