University of Jos Institutional Repository >
Medical Sciences >
Medical Laboratory Sciences >

Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/1699

Title: Evaluation of the Contribution of Major T Cell Subsets to IFN-ᵣ Production in TB Infection by ELISPOT
Authors: Lugos, Moses D.
Adetifa, Ifedayo M. O.
Donkor, Simon
Hill, Philip C.
Adegbola, Richard A.
Ota, Martin O. C.
Keywords: Tuberculosis
T lymphocyte subsets
Issue Date: 2009
Publisher: Immunological Investigations
Series/Report no.: Vol. 38;Pp 341–349
Abstract: Interferon gamma remains a key effector molecule that is still widely used as the most informative biomarker for screening human immune responses against tuberculosis, particularly in ELISPOT assays. We investigated the participation of CD4+ and CD8+ T lymphocytes in the PBMC responses to Mycobacterium tuberculosis (Mtb) specific antigens in 33 TB cases and 49 contacts. Responses to ESAT-6 were higher than CFP- 10. There was no significant difference in responses to both Mtb antigens between cases and contacts. PBMCs response to ESAT-6 but not CFP-10 in cases was significantly reduced by depletion of CD4+ cells whereas CD8+ cell depletion had no impact. In conclusion, ESAT-6 is a more recognized antigen in this population, and CD4+ lymphocytes are the main participants in IFN-g response by ELISPOT. Thus, a decline of CD4+ T lymphocytes below a critical level might affect the sensitivity of IFN-g release assays for detecting Mtb infection.
URI: http://hdl.handle.net/123456789/1699
ISSN: 1532-4311
Appears in Collections:Medical Laboratory Sciences

Files in This Item:

File Description SizeFormat
evaluation of the contribution ....pdf162.82 kBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback