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|Title: ||Evaluation of the Contribution of Major T Cell Subsets to IFN-ᵣ Production in TB Infection by ELISPOT|
|Authors: ||Lugos, Moses D.|
Adetifa, Ifedayo M. O.
Hill, Philip C.
Adegbola, Richard A.
Ota, Martin O. C.
T lymphocyte subsets
|Issue Date: ||2009|
|Publisher: ||Immunological Investigations|
|Series/Report no.: ||Vol. 38;Pp 341–349|
|Abstract: ||Interferon gamma remains a key effector molecule that is still widely used as the most
informative biomarker for screening human immune responses against tuberculosis,
particularly in ELISPOT assays. We investigated the participation of CD4+ and CD8+
T lymphocytes in the PBMC responses to Mycobacterium tuberculosis (Mtb) specific
antigens in 33 TB cases and 49 contacts. Responses to ESAT-6 were higher than CFP-
10. There was no significant difference in responses to both Mtb antigens between
cases and contacts. PBMCs response to ESAT-6 but not CFP-10 in cases was significantly
reduced by depletion of CD4+ cells whereas CD8+ cell depletion had no impact.
In conclusion, ESAT-6 is a more recognized antigen in this population, and CD4+ lymphocytes
are the main participants in IFN-g response by ELISPOT. Thus, a decline of
CD4+ T lymphocytes below a critical level might affect the sensitivity of IFN-g release
assays for detecting Mtb infection.|
|Appears in Collections:||Medical Laboratory Sciences|
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