Isolation and Characterization of the Chemical Structure of Compound in Methanol Extract of Cochlospermum planchonii Hook.F (Cochlospermaceae)

Abstract

Cochlospermum planchonii Hook.F (Cochlospermaceae) leaf is used by the Hausa/Fulani in the Northern Nigeria for the management of pain, inflammation and related diseases yet, its chemistry is poorly known. The present study is to isolate and characterize the structure (s) of compound (s) in methanol extract of Cochlospermum planchonii. Fractions of methanol samples were fractionated using TLC method into 80 fractions. Based on TLC-DPPH assay, similar fractions were pooled together by Preparative TLC. Aromatic singlet proton at δ 8.03 in the 'H NMR spectrum indicates that the compound might be an isoflavone. The final structure of the compound was confirmed by comparing the NMR data of the compound with the literature data. The NMR spectrum was obtained in methanol- δ4 and the literature values were obtained in DMSO. Keywords: Cochlospermum planchonii, Isolation, Characterization, Singlet proton