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|Title: ||Erythrocyte Osmotic Fragility and Lipid Peroxidation Following Chronic Co-Exposure of Rats to Chlorpyrifos and Deltamethrin, and the Beneficial Effect of Alpha-Lipoic Acid|
|Authors: ||Uchendu, Chidiebere|
Ambali, Suleiman F.
Ayo, Joseph O.
Esievo, King A.N.
Umosen, Angela J.
|Keywords: ||Oxidative stress|
|Issue Date: ||2014|
|Publisher: ||Science Direct|
|Series/Report no.: ||Vol. 1;Pp 373–378|
|Abstract: ||The present study aimed to evaluate the effect of chronic co-exposure to chlorpyrifos (CPF)
and deltamethrin (DLT) on erythrocyte osmotic fragility, lipid peroxidation and the ameliorative
effect of alpha-lipoic acid (ALA) on erythrocyte fragility. Thirty-six male Wistar
rats divided into six groups of six rats each were used for the study. Groups I (S/oil) and II
(ALA) were given soya oil (2 ml/kg) and ALA (60 mg/kg), respectively. Rats in group III (DLT)
and IV (CPF) were exposed to DLT (6.25 mg/kg) and CPF (4.75 mg/kg) (1/20th of the previously
determined LD50 of 125 mg/kg and 95 mg/kg, respectively, over a period of 48 h).
Rats in group V (CPF + DLT) were co-exposed to CPF (4.75 mg/kg) and DLT (6.25 mg/kg),
while those in group VI (ALA + CPF + DLT) were pretreated with ALA (60 mg/kg) and then
co-exposed to CPF and DLT, 45 min later. The treatments were administered by gavage once
daily for a period of 16 weeks. Blood collected at the end of the experimental period were
analyzed for erythrocyte osmotic fragility and malondialdehyde (MDA) concentration. The
study showed that chronic co-exposure to CPF and DLT resulted in an increase in erythrocyte
fragility and MDA concentration which were ameliorated by supplementation with
alpha-lipoic acid. The study concluded that repeated co-exposure to CPF and DLT elevated
erythrocyte fragility probably due to increased lipid peroxidation, and pretreatment with
alpha-lipoic acid ameliorated these alterations.|
|Appears in Collections:||Veterinary Physiology, Biochemistry and Pharmacology|
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