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Title: | Antibacterial Potency of the Combined Extracts of Ocimum Gratissimum and Antibiotics against Salmonella Typhi and Salmonella Paratyphi |
Issue Date: | 2016 |
Publisher: | Asian Journal of Science and Technology |
Series/Report no.: | Vol. 07;Iss. 8; Pp 3375-3379 |
Abstract: | The study assessed the antibacterial effect of the extracts of Ocimumgratissimum and their combined
effect with antibiotic drugs against Salmonella Typhi and Salmonella Paratyphi respectively. The
extract of Ocimumgratissimum were prepared using Soxhlet apparatus for ethanol and petroleum ether
extract, and hot water percolation for aqueous extracts. The antibacterial activities of the plant extracts,
the inhibitory effect to some antibiotic drugs; Ciprofloxacin, Gentamycin, Rifampicin, Erythromycin,
Chloramphenicol and Ampiclox as well as their combined effect with the antibiotics were evaluated
using the disk diffusion method. The inhibitory zones were recorded in millimeters. Phytochemical
analysis of the extract were as well carried using standard procedure. The result of this study showed
that the hot water extract showed higher antibacterial activities against S. Typhi and S. Paratyphi
respectively, followed by the ethanol extract. The petroleum extract however had the least antibacterial
effect at the concentration used. All the antibiotic used had greater inhibitory activities on the test
organisms except chloramphenicol that showed partial inhibition against S. Typhi. The result also
showed that the combined effect of the plant extracts and antibiotics used showed additive interaction
against the test isolates. The result of the phytochemical analysis revealed the presence of some
secondary metabolites such as flavonoids, carbohydrates, tannins, alkaloids and steroids and terpenes.
The findings of this study therefore validate the antibacterial properties of Ocimumgratissimum and its
possession of some active compounds as well as its concomitant use with conventional antibiotics to
treat many bacterial infections. It is recommended that the in vivo toxicological activity of the plant be
evaluated to determine the real effectiveness and potential toxic effects. |
URI: | http://hdl.handle.net/123456789/2115 |
ISSN: | 0976-3376 |
Appears in Collections: | Microbiology
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