Characterization and Phytochemical Screening of nHexane Oil Extract from Cissus aralioides Seeds

Abstract

The aim of this research work is to investigate the physico-chemical properties and phytochemical screening of cissus aralioides oil using solvent extraction method, with n-hexane as the extracting solvent. The Phytochemical analysis was determined using GC-MS, which reveal the presences of Diethyl Phthalate; 6-Octadecenoic acid and Octadecanoic acid, 2-(2-hydroxyethoxy) ethyl ester. The phytochemical screening shows a positive result for alkaloids and steroids. The AOAC method of Analysis was employed in the determination of the physico-chemical properties of the oil. The chemical properties of the oil determined include saponification value, free fatty acid, iodine value, peroxide value and acid value. The physical properties of the oil determined are specific gravity, refractive index, cold test. The values obtained are; Saponification value (130.58 ± 0.30mgKOH/g]]free fatty acid (2.13 ± 0.025mgKOH/g), iodine value (80.74 ± 1.53gI/100g), peroxide value (34.67 ± 0.10mEq/kg), acid value (4.24mgKOH/g), Relative density (0.9220),Refractive index at 25 ◦C (1.4758 ± 0.0002) , and cold test (Low Turbidity). The moisture content of the seeds was 25.1 ± 0.18 and the oil yield was 23 ± 0.55. From the results obtained it can be seen that cissus aralioides oil, has a great potential in cosmetic, soap and pharmaceutical industries.