Relevance of Serum LH/FSH/Prolactin and Anti Mullerian Hormone Estimation in Invitro Fertilization

Abstract

Background: Infertility has been considered a field of importance in public health circles due to its high prevalence and its social effects on affected couples and families. Detection and evaluation of incidence of infertility and prevalence in a population is achieved through surveillance which includes determination of the different types of infertility clinically and the use of various laboratory investigations for its detection and treatment. Aim: This study aimed at evaluating the relevance of serum AMH, LH, FSH and Prolactin measurement in women seeking invitro fertilization in our environment. Methodology: This was a cross sectional prospective study were women assessing invitro fertilization (IVF) intervention at Kauna Specialist Hospital, Jos, Nigeria were enrolled. Demographic data was collected using self administered questionnaires with special attention focused on known history of female infertility. 10 mls of venous blood was collected from each subject, into plain containers and allowed to clot. The samples were analyzed for hormone concerntrations using enzyme immunoassay (EIA), according to the World Health Organization (WHO) matched reagent programme protocol (manual) for EIA kits (protocol/version of December 1998 for Prolactin, LH, FSH; and protocol 09.3.1 for AMH).Data generated were analysed using SPSS version 21 for simple statistical analysis and comparism of means between groups, p<0.05 was considered significant. Results: A total of 124 infertile women seeking invitro fertilization due to different etiology of infertility were monitored in the course of their treatment. The positive pregnant women had a mean serum AMH of 11.61ng/ml,SD=13.896 while the non pregnant women had mean =27.5ng/ml5, SD=43.919, p=0.003) A pearson correlation(r=-0.201,p=0.025) was obtained for pregnancy outcome following IVF and serum AMH level. In addition, r=0.705(p=0.000) was obtained when serum FSH and LH were compared for possible association based on IVF success. Conclusion: Our study suggests that serum AMH levels decreased steadily with increasing age in cases of infertility. In addition, Serum FSH and LH had a positive correlation which may imply that they are good predictors of ovarian reserve.Mesurement of serum AMH, LH, FSH and Prolactin are relevant for evaluation of possible IVF success rate.