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|Title: ||Laboratory Diagnosis of Pulmonary Tuberculosis: A Comparison of Different Methods.|
|Authors: ||Mawak, J.D|
|Issue Date: ||2001|
|Publisher: ||Science forum: J. Pure and Appl. Sci .|
|Series/Report no.: ||Vol.4;No.2;Pp 244-250|
|Abstract: ||A laboratory study designed to compare the sensitivity of microscopy (direct and concentrated
smears): Direct and Concentrated sputum culture as well as comparing Ziehl-Neelsen and
Auramine-Phenol staining methods in the diagnosis or pulmonary tuberculosis was carried out.
The survey involved one hundred and Eighty Six (180) new patients seen at the chest and General
Out-patient clinics of the Jos Universitv Teaching Hospital (JUTH) and the out-patient
Department of the Evangel Hospital Jos. with symptoms of broncho-pulmonary disorders and
who were sent to the Microbiology Laboratory for Acid fast bacilli (AFB) Microscopy between
February, 1996 and November. I<)1)7. Also included were one hundred and thirty (130) sputum
smears made from different samples sent to the laboratory Within the same period. A total of 55
(29.57%) patients were positive for acid fast bacilli by both microscopy and culture methods.
Thirty-eight, 38 (69.1 %) of the 55 positive cases were detected by direct smear. 43 (78.2%) by
concentrated smear, 16 (29.1 %) by direct sputum culture and 33 (60.0%) by concentrated sputum
culture. The difference between a single concentrated smear, 78.2% and 3 consecutive direct
smears (69.1 %) for identifying tubercle bacilli was not statistically significant (P>0.05) but was
significantly different from 60.0% for concentrated culture and 28.57% direct culture (P<0.05).
Concentrated sputum smear examination for routine diagnosis of pulmonary tuberculosis is
|Appears in Collections:||Medical Microbiology|
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