Browsing by Author "Patricia MankoLar"

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    Prevalence and drug-resistance profile of plasmid borne extended spectrum beta-lactamase (ESBLs) resistance genes in multidrug resistant Escherichia coli from HIV-1 positive individuals in Jos, Nigeria
    (2020-09-10) Murna Ahmed Ali; Ocheme Julius Okojokwu; Ujeh Anthony Augustine; Chad Achenbach; Joseph AjeAnejo-Okopi; Patricia MankoLar; Godwin Imade; Atiene Solomon Sagay
    Production of extended-spectrum beta-lactamases (ESBLs) can lead to treatment failures when the antibiotics are used. This study detected ESBLs genes on Multidrug Resistant Escherichia coli from HIV-infected individuals attending some hospitals in Jos. Eighty (80) isolates of multidrug resistant (MDR) E. coli were screened for plasmids. ESBLs genes including bla-CTX, bla-TEM and bla-SHV were detected on the plasmids using polymerase chain reaction (PCR) with 100 bp DNA ladder as DNA molecular weight marker. Out of the total 38 plasmids tested, ESBLs genes occurred in 13 (34.21%), with the bla-TEM dominating [7 (53.85%)] over the bla-CTX [4 (30.77%)]. Two (15.38%) of the isolates carried both genes. None of the isolates carried the bla-SHV gene in our study. All the strains showed resistance to SXT, AMC and CTX. Resistance was most frequently observed against SXT (13), AMC (13), CTX (13), CFM (12), F (8), NA (6), GN (5), CRO (4), OFX (2) and CIP (1). None of the ESBLs-bearing strains showed resistance to IPM. Result showed 34.21% prevalence of ESBLs and suggested the need to be more cautious with the clinical use of third generation Cephalosporins especially for the treatment of acute infections caused by E. coli due to the high resistance recorded.